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This Concept Map has information related to: Lab 3 flow chart, e. Place the 0, Gas Sensor into the bottle as shown in Figure I. Gently push the sensor down into the bottle until it stops. The sensor is designed to seal the bottle without the need for unnecessary forc ???? f. When 30 seconds has passed, Click II~ Collect I to begin data collection., 13. Repeat Step 8 and record the reaction rate for each data set in Table 4. Calculate and record the 4verage rate in Table 4. ???? 14. Record the average rate and the temperature of your water bath from Table 4 on the class data table. When the, 6. Move your data to a stored run. To do this, choose Store Latest Run from the Experiment menu ???? 7. Collect data for test tubes 2 and 3:, 3. Place three test tubes in a rack and label them 1,2, and 3. Fill each test tube with 3 mL of3.0% !H,O, and 3 mL of water. ???? 4. Initiate the enzyme catalyzed reaction., • Add 10 Drops of the enzyme solution to test tube 1. Repeat Steps 4 - 6. • Add 10 drops of the enzyme solution to test tube 2. Repeat Steps 4 - 6. • Add 10 drops ofthe enzyme solution to test tube 3. Repeat Steps 4 - 5. ???? 13. Repeat Step 8 and record the reaction rate for each data set in Table 4. Calculate and record the 4verage rate in Table 4., 8. Using the mouse, select the initial linear region of your data on the graph. Click on the Linear Flt button, I!;J. record the value of the slope, m, for each of the three solutions. (The linear regression statistics jrre displayed in a floating box for each of the data sets.) ???? 9. To print a graph of concentration vs. volume showing all three data runs:, 2. Connect the Oxygen Gas Sensor to the computer interface. Prepare the computer for data collection by opening the file "06A Enzyme (02)" from the Biology with Computers folder of Logger Pro. ???? 3. Place three test tubes in a rack and label them 1,2, and 3. Fill each test tube with 3 mL of3.0% !H,O, and 3 mL of water., 19. Using the test tube labeled pH 10, add 10 drops of enzyme solution and repeat Steps 4 - 5. ???? 20. Repeat Steps 8 and 9 to calculate the rate of reaction and print your graph. Record the reaction rate for each pH value in Table 6., IS. Place three clean test tubes in a rack and label them pH 4, pH 7, and pH 10. ???? 16. Add 3 mL of3% H,O, and 3 mL ofa pH buffer to each test tube, as in Table I., 17. Using the test tube labeled pH 4, add 10 drops of enzyme solution and repeat Steps 4 - 6. ???? 18. Using the test tube labeled pH 7, add 10 drops of enzyme solution and repeat Steps 4 - 6.